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A mycofactocin-associated dehydrogenase is essential for ethylene glycol metabolism by Rhodococcus jostii RHA1
Tetsu Shimizu1, Kai Suzuki2, Masayuki Inui3,4
1Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, Kizugawa-Shi, Kyoto, 619-0292, Japan.
Applied Microbiology and Biotechnology
|January 4, 2024
Summary
Rhodococcus jostii RHA1 utilizes ethylene glycol as its sole carbon source, assimilating it via glycolate. A novel dehydrogenase, EgaA, and mycofactocin are crucial for this process, impacting other alcohol metabolisms.
Area of Science:
- Microbiology
- Biochemistry
- Metabolic Engineering
Background:
- Ethylene glycol is a key industrial chemical and polymer precursor.
- Understanding microbial metabolism of industrial chemicals is vital for biotechnology.
- Rhodococcus jostii RHA1 is known for its complex metabolic capabilities.
Purpose of the Study:
- To investigate the metabolic pathway of ethylene glycol utilization in Rhodococcus jostii RHA1.
- To identify the key enzymes and genes involved in ethylene glycol assimilation.
- To explore the broader role of identified enzymes in the metabolism of other alcohols.
Main Methods:
- Growth experiments using ethylene glycol as the sole carbon source.
- Gene deletion analysis to determine gene function.
- Transcriptome sequencing to identify differentially expressed genes.
- Biochemical assays to confirm enzyme activity and interactions.
Main Results:
- Rhodococcus jostii RHA1 can grow using ethylene glycol as the sole carbon and energy source.
- Ethylene glycol assimilation occurs via glycolate, with a putative glycolate dehydrogenase being essential.
- A mycofactocin-associated dehydrogenase (EgaA) is critical for ethylene glycol assimilation and also affects the metabolism of other alcohols.
- Mycofactocin (MFT) acts as the physiological electron acceptor for EgaA.
- A major aldehyde dehydrogenase (RHA1_ro06081) plays a significant role in ethylene glycol metabolism.
Conclusions:
- Rhodococcus jostii RHA1 possesses a unique pathway for ethylene glycol assimilation involving glycolate and mycofactocin.
- EgaA is a key enzyme in ethylene glycol metabolism and has a broader role in alcohol utilization.
- This study elucidates novel metabolic capabilities of Rhodococcus jostii RHA1 with potential applications in bioremediation and biotransformation.
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