High-sensitivity flip chip blue Mini-LEDs miniaturized optical instrument for non-invasive glucose detection

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Spectroscopy

Background:

• Traditional colorimetric glucose detection relies on peroxidase enzymes, facing challenges in purification, storage, and portability of optical instruments.
• Existing chemical methods for glucose detection are often time-consuming, requiring steps like centrifugation and purification.

Purpose of the Study:

• To develop a novel, enzyme-free colorimetric method for glucose detection.
• To design and validate a miniaturized optical instrument (FC blue Mini-LEDs MOI) for portable glucose monitoring.
• To improve the sensitivity, stability, and efficiency of glucose detection systems.

Main Methods:

• A new detection solution was prepared using ammonium metavanadate and sulfuric acid, eliminating the need for peroxidase.
• The effect of detection solution concentration on absorbance sensitivity was analyzed.
• A flip chip blue Mini-LEDs miniaturized optical instrument (FC blue Mini-LEDs MOI) was designed, incorporating fiber optics, collimating lenses, a spectrometer, and a blue Mini-LED (459 nm).

Main Results:

• The developed MOI demonstrated a linear detection range for glucose from 0.1-10 mM with a regression equation y = 0.0941x + 0.1341 (R² = 0.9744).
• The limit of detection was 2.15 mM and the limit of quantification was 7.163 mM.
• The detection solution preparation time was reduced to 10 min, with a 2.3-fold increase in absorbance sensitivity and stability over 14 days at 3 °C (0.6% absorbance decrease).

Conclusions:

• The proposed method offers an enzyme-free, rapid, and sensitive approach for glucose detection.
• The miniaturized optical instrument provides a portable, stable, and quantifiable glucose monitoring system.
• This technology has potential applications in blood glucose monitoring and non-invasive detection in saliva and tear fluids.