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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Related Experiment Video

Updated: Jul 6, 2025

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
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Live cell pool and rare cell isolation using Enrich TROVO system.

Stephen Rotatori1, Yichong Zhang1, Kirby Madden-Hennessey1

  • 1Enrich Biosystems Inc., 21 Business Park Drive. STE. 4, Branford, CT 06405, USA.

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|January 4, 2024
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Summary

The novel Enrich TROVO system offers clog-resistant, nonfluidic live cell isolation. This technology achieves high recovery and viability, significantly enriching rare cells for downstream applications.

Keywords:
Cell sortingCollagenaseGelMA-hydrogelImagingNonfluidicPhoto-crosslinkingRare cell enrichmentSingle cell multi-omicsViability

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Existing cell isolation technologies often suffer from clogging and reduced cell viability.
  • Efficient isolation of live cells from heterogeneous samples is crucial for various biological and medical applications.

Purpose of the Study:

  • To develop a novel, nonfluidic cell isolation system that overcomes limitations of current technologies.
  • To demonstrate the efficacy of the Enrich TROVO system in capturing and enriching live cells with high viability and purity.

Main Methods:

  • The TROVO system integrates imaging-based cell selection with photo-crosslinking of gelatin methacrylate (gelMA) hydrogels for cell capture.
  • Captured cells are released via enzymatic hydrogel digestion for downstream analysis or culturing.

Main Results:

  • The system achieves a 48% cell recovery rate with 90% viability.
  • Rare cells are enriched 506-fold with 93% efficiency from a 1:10^4 mixture.
  • Enrichment ratios up to 9128 are possible, with subsequent negative isolation yielding 100% purity and 49% recovery.

Conclusions:

  • The TROVO system provides a clog-resistant, biocompatible, and versatile solution for live cell isolation.
  • This technology significantly advances cell sorting capabilities, particularly for rare cell enrichment.
  • The TROVO system is suitable for processing diverse sample sizes and enables downstream applications.