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Related Experiment Videos

The membrane attack complex of complement induces permeability changes via thresholds in individual cells.

A K Patel, A K Campbell

    Immunology
    |January 1, 1987
    PubMed
    Summary

    Complement attack creates cell permeability and lysis thresholds in polymorphonuclear leucocytes (PMN). Adenosine inhibits membrane attack complex removal, increasing PMN lysis.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • The complement system plays a crucial role in innate immunity.
    • The membrane attack complex (MAC) is a key effector of complement-mediated cell lysis.

    Purpose of the Study:

    • To establish the existence of permeability and lytic thresholds in individual rat polymorphonuclear leucocytes (PMN) attacked by MAC.
    • To investigate the dynamics of cell recovery and lysis following complement activation.

    Main Methods:

    • Flow cytometry was utilized to quantify propidium iodide fluorescence in rat PMN.
    • Cells were exposed to the membrane attack complex (MAC) following complement pathway activation.

    Main Results:

    • Approximately 30-40% of PMN became permeable to molecules after MAC attack.

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  • Three distinct PMN populations were identified based on recovery status relative to permeability and lytic thresholds.
  • Individual PMN exhibited variable timing for permeability (7.5 ± 2.5 min) and lysis (11.5 ± 1.0 min) thresholds.

    • Conclusions:

    • Individual PMN display distinct permeability and lysis thresholds following complement attack.
    • Adenosine inhibited MAC removal, leading to increased PMN permeability and lysis.