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Related Concept Videos

Mass Spectrometry: Molecular Fragmentation Overview01:20

Mass Spectrometry: Molecular Fragmentation Overview

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The ionization of a molecule into a molecular ion inside the mass spectrometer causes instability in the molecule's structure due to the loss of an electron. This eventually leads to the fragmentation or breaking of some bonds in the molecule. The fragmentation occurs predominantly at specific bonds to yield relatively stable fragments.
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can...
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¹³C NMR: ¹H–¹³C Decoupling01:04

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The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
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Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
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"Covalent-Disassembly"-Based Approaches For Sensing Applications.

F Zelder1

  • 1Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057, Zurich, Switzerland.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|January 5, 2024
PubMed
Summary

This study introduces covalent-disassembly probes for detecting various analytes like polyoxophosphates and HCN. These probes offer a novel approach for chemical and biological sensing applications.

Keywords:
activity-based sensingdesign principlesinduced-fit“covalent-assembly““covalent-disassembly“

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Area of Science:

  • Analytical Chemistry
  • Supramolecular Chemistry
  • Chemical Biology

Background:

  • Analyte detection is vital across chemical, medicinal, environmental, and biological fields.
  • Traditional methods often rely on molecular recognition and bond formation.
  • Bond breakage reactions offer an underexplored avenue for analyte detection.

Purpose of the Study:

  • Introduce metal-salen and metal-imine complexes as covalent-disassembly (DB)-probes.
  • Explore the construction and application of DB-probes for diverse analytes.
  • Present design principles for developing novel DB-probes.

Main Methods:

  • Utilized metal-salen and metal-imine complexes as DB-probes.
  • Investigated the role of molecular building blocks in probe design.
  • Demonstrated analyte detection in live cells and foodstuff.
  • Detailed the disassembly mechanism of a Fe(III)-salen probe.

Main Results:

  • DB-probes successfully detected polyoxophosphates, thiols, amino acids, HCN, and pH changes.
  • Achieved high selectivity for pyrophosphate detection via a multistep disassembly process.
  • Demonstrated an 'induced-fit' principle for enhanced metal complex structural changes.
  • Highlighted the importance of structural functionalization in probe development.

Conclusions:

  • Covalent-disassembly offers a powerful strategy for analyte sensing.
  • DB-probes demonstrate broad applicability in complex biological and food matrices.
  • The 'induced-fit' mechanism provides a pathway for highly selective analyte detection.
  • Summarized design principles will guide future development of advanced sensing systems.