Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

10.9K
Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
10.9K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

6.9K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.9K
The Contractile Ring02:15

The Contractile Ring

6.4K
Contractile rings are composed of microfilaments and are responsible for separating the daughter cells during cytokinesis. Contractile ring assembly proceeds along with other cell cycle events; however, very few mechanistic details are known about the timing and coordination of the contractile rings with the cell cycle.
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...
6.4K
Experimental RNAi02:15

Experimental RNAi

6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
Spermatogenesis01:41

Spermatogenesis

102.5K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.5K
Meiosis I03:09

Meiosis I

40.7K
Meiosis is the division of a diploid cell into haploid cells forming sperm and eggs in animals through differentiation. Meiosis I is the first stage of meiosis, where the genetic recombination of homologous chromosomes and the reduction of the ploidy level by half occurs.
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
40.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Promoting contraceptive use among unmarried female migrants in one factory in Shanghai: a pilot workplace intervention.

BMC health services research·2007
Same author

Apoptosis related protein 3, an ATRA-upregulated membrane protein arrests the cell cycle at G1/S phase by decreasing the expression of cyclin D1.

Biochemical and biophysical research communications·2007
Same author

Efficient one-pot synthesis of highly substituted pyridin-2(1H)-ones via the Vilsmeier-Haack reaction of 1-acetyl,1-carbamoyl cyclopropanes.

Organic letters·2007
Same author

Effects of Andrographitis Paniculata extracts on the expression of CD40 in endothelial cells.

Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban·2007
Same author

Theoretical and experimental investigations on the size of alginate microspheres prepared by dropping and spraying.

Journal of microencapsulation·2007
Same author

Beneficial effects of losartan on vascular injury induced by advanced glycosylation end products and their receptors in spontaneous hypertension rats.

Molecular and cellular biochemistry·2007

Related Experiment Video

Updated: Jul 6, 2025

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
07:43

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

12.5K

The RING Domain of Rice HEI10 is Essential for Male, But Not Female Fertility.

Qian Tan1, Xu Zhang1, Qian Luo1

  • 1Joint International Research Laboratory of Metabolic and Developmental Sciences, State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

Rice (New York, N.Y.)
|January 5, 2024
PubMed
Summary
This summary is machine-generated.

A novel rice HEI10 mutation (sh1) causes male sterility but partial female fertility. This shorter HEI10 protein, lacking its RING domain, offers insights into differential gamete development and meiosis.

Keywords:
FertilityGameteHEI10HeterochiasmyMeiosisRING domainRice

More Related Videos

In Vivo Visualization of Calcium Transients during Fertilization and Early Development in C. elegans
05:11

In Vivo Visualization of Calcium Transients during Fertilization and Early Development in C. elegans

Published on: July 12, 2024

386
Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis
10:31

Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis

Published on: May 10, 2019

20.0K

Related Experiment Videos

Last Updated: Jul 6, 2025

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
07:43

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

12.5K
In Vivo Visualization of Calcium Transients during Fertilization and Early Development in C. elegans
05:11

In Vivo Visualization of Calcium Transients during Fertilization and Early Development in C. elegans

Published on: July 12, 2024

386
Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis
10:31

Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis

Published on: May 10, 2019

20.0K

Area of Science:

  • Genetics
  • Molecular Biology
  • Plant Science

Background:

  • HEI10 is a crucial E3 ubiquitin ligase for meiosis and gamete development in both sexes.
  • Null mutations in HEI10 lead to complete sterility, highlighting its essential role.

Purpose of the Study:

  • To investigate a novel rice HEI10 allele, sh1, with distinct fertility effects.
  • To understand the molecular basis of differential male and female fertility in sh1 mutants.

Main Methods:

  • Identification and characterization of the sh1 mutant allele in rice.
  • Genetic analysis including allelic tests and complementation assays.
  • Localization and interaction studies of the truncated HEI10 protein.

Main Results:

  • The sh1 mutation results in a truncated HEI10 protein lacking the N-terminal RING domain due to a large inversion.
  • sh1 exhibits complete male sterility but retains partial female fertility, unlike null alleles.
  • Truncated HEI10 localizes to the nucleus and partially rescues female fertility in null mutants.

Conclusions:

  • The sh1 allele provides a unique model for studying HEI10 function and its differential impact on male and female meiosis.
  • This finding offers new tools to explore the distinct mechanisms governing male and female gamete development.