Strain-specific antigens of Toxoplasma gondii

A detailed immunological assessment of strain-specific antigens of Toxoplasma gondii has not been reported. We developed rabbit antisera against three strains of toxoplasma obtained from divergent sources. These strains included the frequently studied laboratory strain RH, strain C, obtained from a naturally infected kitten, and strain P, which is maintained by passage in mice. The rabbit antisera were used to identify unique strain-specific and commonly shared tachyzoite antigens by radioiodination followed by immunoprecipitation and Western blot analysis. Both qualitative and quantitative differences of a number of the major tachyzoite antigens were found in these assays. A parasite plaque reduction assay using parasiticidal monoclonal antibody showed marked differences in the ability to kill these three different tachyzoite subtypes, further supporting antigenic variation among T. gondii strains.