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Related Experiment Video

Updated: Jul 6, 2025

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A programmable reaction-diffusion system for spatiotemporal cell signaling circuit design.

Rohith Rajasekaran1, Chih-Chia Chang2, Elliott W Z Weix3

  • 1Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA; Integrated Program in Biochemistry Graduate Program, University of Wisconsin-Madison, Madison, WI 53706, USA.

Cell
|January 5, 2024
PubMed
Summary

Scientists engineered programmable protein circuits in mammalian cells using bacterial MinD and MinE (MinDE) proteins. These circuits create frequency-barcoded signals for visualizing and controlling cellular activities.

Keywords:
AM/FM single-cell reportersdigital signal processingprotein condensatesprotein oscillationsreaction-diffusion systemsspatiotemporal signaling circuitssynthetic biology

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Area of Science:

  • Synthetic Biology
  • Cellular Engineering
  • Biophysics

Background:

  • Cells utilize complex spatiotemporal signaling for biological functions.
  • Current synthetic strategies for engineering cellular spatiotemporal dynamics are limited.

Purpose of the Study:

  • To develop a programmable reaction-diffusion platform for engineering protein oscillations, patterns, and circuits in mammalian cells.
  • To enable precise control and visualization of cellular activities at critical length and timescales.

Main Methods:

  • Utilized bacterial MinD and MinE (MinDE) proteins to create programmable reaction-diffusion circuits.
  • Engineered protein-protein interactions to link circuit dynamics with cellular functions.
  • Employed digital signal processing for analyzing frequency-barcoded fluorescence signals.

Main Results:

  • Demonstrated MinDE circuits function as 'single-cell radios' emitting frequency-barcoded signals.
  • Constructed reporter circuits to visualize endogenous cell signaling dynamics.
  • Built control circuits to synthetically pattern cellular activities like protein condensate assembly and actin filamentation.

Conclusions:

  • Established a novel paradigm for engineering spatiotemporal signaling in mammalian cells.
  • Enabled visualization, probing, and synthetic control of cellular activities.
  • Opened new avenues for understanding and manipulating cell biology.