Construction and Application of a Pepsin-Functionalized Covalent Organic Framework with Prominent Chiral Recognition
Jiale Zhang1,2, Xue Liu1,2, Qixuan Mu1,2
1Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, 210009, China.
Chemistry (Weinheim an Der Bergstrasse, Germany)
|January 5, 2024
Abstract:
The stable Pepsin@covalent organic framework (Pepsin@COF) were constructed base on matching COF pore diameter to pepsin dimension. It exhibits excellent chiral recognition capabilities (e. e. % up to 62.63 %) and potential for enantioseparation. Furthermore, a positive correlation between the immobilized enzyme activity and chiral recognition was revealed, offering insights for the design of biocatalytic nanosystems in chiral separation.


