Related Experiment Video
Updated: Jul 6, 2025
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Serum circPRDM5 as a novel diagnostic biomarker for acute myocardial infarction
Ruoyu Liu1, Lijuan Hu1, Yun Zhou2
1Department of Clinical Laboratory, China-Japan Friendship Hospital (Institute of Clinical Medical Sciences), Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Insights
Circular RNA PRDM5 (circPRDM5) shows promise as a novel biomarker for acute myocardial infarction (AMI). Lower serum circPRDM5 levels in AMI patients, combined with other markers, improve diagnosis.
Area of Science:
- Biochemistry
- Molecular Biology
- Cardiovascular Research
Background:
- Circular RNAs (CircRNAs) are implicated in cardiovascular diseases.
- The diagnostic potential of circPRDM5 in acute myocardial infarction (AMI) remains unexplored.
Purpose of the Study:
- To investigate circPRDM5 as a novel biomarker for AMI.
- To assess the diagnostic value of serum circPRDM5 in AMI patients.
Main Methods:
- Bioinformatic screening identified circPRDM5.
- Agarose gel electrophoresis and Sanger sequencing confirmed primers.
- Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) measured serum circPRDM5 levels.
- Receiver operating characteristic (ROC) curve analysis evaluated diagnostic accuracy.
Main Results:
- Serum circPRDM5 expression was significantly lower in AMI patients compared to healthy controls and angina patients (P < 0.001).
- The ROC curve analysis yielded an area under the curve of 0.862 for serum circPRDM5.
- Combining circPRDM5 with cardiac troponin T (cTnT) and creatine kinase-MB (CK-MB) enhanced AMI diagnostic sensitivity.
- Serum circPRDM5 levels increased post-percutaneous coronary intervention (PCI).
Conclusions:
- CircPRDM5 serves as a novel biomarker for AMI.
- The combined use of circPRDM5, cTnT, and CK-MB improves the diagnostic efficacy for AMI.
Background:
Circular RNA (CircRNA) is known to play an important role in cardiovascular diseases, but its use as a biomarker of acute myocardial infarction (AMI) has not been studied. This study explores the feasibility of circPRDM5 as a novel biomarker of AMI.
Methods:
CircPRDM5 was screened by bioinformatics, the correct circPRDM5 primers were tested by agarose gel electrophoresis (AGE) and Sanger sequencing, and the expression level of serum circPRDM5 was detected by Quantitative Reverse Transcription-Polymerase Chain Reaction. (qRT-PCR), and the diagnostic value of circPRDM5 was analyzed by the receiver operating characteristic (ROC) curve.
Results:
The expression of circPRDM5 in serum of AMI patients was significantly decreased compared with that of healthy control group and angina group (P < 0.001). The area under ROC curve of serum circPRDM5 was 0.862 [95 % CI, 0.814-0.909]. The combined diagnosis of serum circPRDM5, cardiac troponin T (cTnT) and creatine kinase-MB (CK-MB) could improve the sensitivity of diagnosing AMI. The expression level of serum circPRDM5 increased after percutaneous coronary intervention (PCI).
Conclusions:
CircPRDM5 can be used as a novel biomarker for AMI, and its combination with cTnT and CK-MB can improve diagnostic value.
More Related Videos
10:12A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay
Published on: August 8, 2013
10:03Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty
Published on: January 28, 2020
Related Concept Videos
01:20Blood Studies for Cardiovascular System I: Cardiac Biomarkers
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
01:19Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...