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Related Experiment Videos

Gluconeogenesis in isolated lamb hepatocytes.

M C Looney, R L Baldwin, C C Calvert

    Journal of Animal Science
    |January 1, 1987
    PubMed
    Summary

    This study quantifies sheep liver gluconeogenesis, revealing kinetic parameters for key substrates like propionate and pyruvate. Glucagon stimulates this process, highlighting its regulation via the adenyl cyclase system.

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    Area of Science:

    • * Ruminant metabolism
    • * Hepatic gluconeogenesis
    • * Biochemical kinetics

    Background:

    • * Understanding gluconeogenesis in ruminants is crucial for managing energy balance.
    • * Kinetic parameters of sheep liver enzymes are not well-established.
    • * Regulation of gluconeogenesis by hormones and substrates requires further investigation.

    Purpose of the Study:

    • * To determine kinetic parameters (Vmax, KS) for sheep hepatocyte gluconeogenesis from various substrates.
    • * To investigate substrate interactions and preferences in glucose synthesis.
    • * To elucidate the regulatory mechanisms of gluconeogenesis in sheep liver.

    Main Methods:

    • * Isolated sheep hepatocytes were used for in vitro metabolic studies.
    • * Enzyme kinetic parameters were estimated using Michaelis-Menten kinetics.
    • * Effects of hormones (glucagon) and substrates on gluconeogenesis were assessed.

    Main Results:

    • * Vmax for propionate, pyruvate, and alanine conversion to glucose were 59.5, 12.8, and 21.5 mol/h/g dry weight, respectively.
    • * KS for propionate and pyruvate were 1 mM and 18–40 μM.
    • * Glucagon stimulated propionate gluconeogenesis, mediated by the adenyl cyclase system.

    Conclusions:

    • * Established kinetic parameters provide a foundation for ruminant metabolic modeling.
    • * Sheep liver gluconeogenesis exhibits substrate preferences and hormonal regulation.
    • * Findings contribute to a deeper understanding of energy metabolism in ruminants.

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