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Updated: Jul 6, 2025
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Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
Published on: April 6, 2017
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Gravity sedimentation reveals functionally and morphologically different platelets in human blood
Erzsébet Ezer1, Diana Schrick1, Margit Tőkés-Füzesi2
1Department of Anesthesiology and Intensive Care, Medical School, University of Pecs, Pecs, Hungary.
Platelets
|January 8, 2024
Summary
Platelet antisedimentation rate (PAR) quantifies platelet flotation, revealing distinct populations with varied structure and function. This method may aid in diagnosing thromboinflammatory disorders.
Area of Science:
- Hematology
- Biophysics
Background:
- Platelets, unlike red blood cells, exhibit flotation in blood under gravity.
- Erythrocyte Sedimentation Rate (ESR) is a known measure, prompting exploration of a platelet analog.
Purpose of the Study:
- To establish and characterize the Platelet Antisedimentation Rate (PAR) as a novel laboratory parameter.
- To investigate the mechanisms and functional implications of platelet flotation.
Main Methods:
- Analysis of platelet count, MPV, IPF, and H-IPF in blood fractions after 1-hour sedimentation.
- Exploration of flotation mechanisms via blood partitioning and time-dependent counts.
- Assessment of platelet structure (EM, AFM) and function (aggregometry).
Main Results:
- Platelet antisedimentation is driven by density and size-exclusion from sedimenting erythrocytes.
- Platelets in upper fractions exhibited increased ADP-induced aggregability and higher H-IPF.
- Electron microscopy revealed larger platelets with more alpha granules in upper fractions.
Conclusions:
- Platelet antisedimentation differentiates platelet populations based on structural and functional properties.
- PAR shows potential as a diagnostic marker for thromboinflammatory disorders.
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