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Published on: January 4, 2011
Antimicrobial Effectiveness Testing of Resorbable Electrospun Fiber Matrix per United States Pharmacopeia (USP)
Emily Sallade1, Derayvia Grimes1, Lily Jeng2
1Clinical Research, Acera Surgical, Inc., St. Louis, USA.
Abstract:
Contamination of surgical, traumatic, and chronic wounds with microorganisms presents a challenge to successful wound healing. In the present in vitro study, a synthetic electrospun fiber matrix (SEFM) cleared for use in the management of chronic, surgical, and traumatic wounds underwent USP (United States Pharmacopeia) <51> Antimicrobial Effectiveness Testing to determine its in vitro effectiveness against various microorganisms commonly found in non-healing wounds. The SEFM was tested in both sheet (s-SEFM) and micronized form (m-SEFM) against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Aspergillus brasiliensis, Candida albicans, Proteus mirabilis, and Enterococcus faecalis. Testing was performed per the USP <51> standard on days 7, 14, and 28. Both the s-SEFM and m-SEFM met the USP <51> acceptance criteria for all microorganisms. The results obtained for s-SEFM demonstrated >1-log10 reduction against E. coli, S. aureus, P. aeruginosa, P. mirabilis, E. faecalis, and C. albicans at day 7; >3-log10 reduction with no detection of these microbes at days 14 and 28, and no increase from initial inoculum at days 7, 14, and 28 against A. brasiliensis. The results obtained for m-SEFM demonstrated >3-log10 reduction with no detectable microorganisms at day 7. The results observed in this study indicate that the SEFM is effective in vitro at inhibiting bacterial and fungal growth and colonization per USP <51> testing.
Insights
A synthetic electrospun fiber matrix (SEFM) effectively inhibited microbial growth in vitro. This wound management material met United States Pharmacopeia standards for antimicrobial effectiveness against common wound pathogens.
Area of Science:
- Biomaterials Science
- Microbiology
- Wound Healing Research
Background:
- Microbial contamination impedes surgical, traumatic, and chronic wound healing.
- Effective antimicrobial strategies are crucial for managing non-healing wounds.
Purpose of the Study:
- To evaluate the in vitro antimicrobial effectiveness of a synthetic electrospun fiber matrix (SEFM).
- To determine SEFM's efficacy against common wound microorganisms per USP <51> standards.
Main Methods:
- Synthetic electrospun fiber matrix (SEFM) in sheet (s-SEFM) and micronized (m-SEFM) forms were tested.
- United States Pharmacopeia (USP) <51> Antimicrobial Effectiveness Testing was performed.
- Efficacy was assessed against *Escherichia coli*, *Pseudomonas aeruginosa*, *Staphylococcus aureus*, *Aspergillus brasiliensis*, *Candida albicans*, *Proteus mirabilis*, and *Enterococcus faecalis* on days 7, 14, and 28.
Main Results:
- Both s-SEFM and m-SEFM met USP <51> acceptance criteria for all tested microorganisms.
- s-SEFM showed significant log reductions, with no microbial detection by day 14 for most tested microbes.
- m-SEFM demonstrated rapid and complete microbial reduction by day 7.
Conclusions:
- The SEFM demonstrates significant in vitro antimicrobial activity against a broad spectrum of wound-associated bacteria and fungi.
- SEFM effectively inhibits microbial growth and colonization, supporting its use in wound management.
