Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Preparation of 1° Amines: Gabriel Synthesis

Preparation of 1° Amines: Gabriel Synthesis

Direct alkylation is not a suitable method for synthesizing amines because it produces polyalkylated products. Gabriel synthesis is the most preferred method to exclusively make primary amines. The method uses phthalimide, which contains a protected form of nitrogen that participates in alkylation only once to predominantly give primary amines.
Strong bases like NaOH or KOH deprotonate the phthalimide to form the corresponding anion, which acts as a nucleophile. Further, the anion attacks an...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Correction: Exendin-4 ameliorates tau hyperphosphorylation and cognitive impairment in type 2 diabetes through acting on Wnt/β-catenin/NeuroD1 pathway.

Molecular medicine (Cambridge, Mass.)·2026

Same author

Synergistic Rh<sub>1</sub>-Cu<sub>1</sub> Dual-Atom-Site Enhancing Performance of Ethane Low-Temperature Oxidation via Auto-Selective Oxygen Source From O<sub>2</sub>/H<sub>2</sub>O.

Angewandte Chemie (International ed. in English)·2026

Same author

Network pharmacology integrated with multi-omics demonstrates that Wumei Wan modulates the progression of colorectal cancer by regulating the focal adhesion-YAP signaling axis.

Journal of ethnopharmacology·2026

Same author

Inositol (1,4,5)-trisphosphate 5-phosphatase promotes survival of uveal melanoma by regulating oncogenic G protein-driven calcium oscillations.

The Journal of biological chemistry·2025

Same author

Directly Oxidizing Ethanol to Glycolic Acid over a Single-Rh-Site Catalyst via Water-Mediated Oxygen Shuttle.

Journal of the American Chemical Society·2025

Same author

The guanine nucleotide exchange factor Ric-8A regulates the sensitivity of constitutively active Gαq to the inhibitor YM-254890.

The Journal of biological chemistry·2025

Search research articles

Related Experiment Video

Updated: Jul 6, 2025

Metal-free Synthesis of Ynones from Acyl Chlorides and Potassium Alkynyltrifluoroborate Salts

Metal-free Synthesis of Ynones from Acyl Chlorides and Potassium Alkynyltrifluoroborate Salts

Published on: February 24, 2015

A Building Block Approach for the Total Synthesis of YM-385781.

Yu Zhu¹, Siyue Liu¹, Johnny Zigmond¹

¹Department of Chemistry, Washington University, St. Louis, MO 63130.

European Journal of Organic Chemistry

|January 8, 2024

Summary

Researchers developed a new synthetic method for creating analogs of Gq/11-signaling pathway inhibitors YM-254890 and FR900359. This approach enables the efficient production of diverse non-natural and labeled compounds for further study.

Keywords:

Cyclic Peptide Synthesis Inhibitors YM-analogs

More Related Videos

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Published on: June 20, 2014

Related Experiment Videos

Last Updated: Jul 6, 2025

Metal-free Synthesis of Ynones from Acyl Chlorides and Potassium Alkynyltrifluoroborate Salts

Metal-free Synthesis of Ynones from Acyl Chlorides and Potassium Alkynyltrifluoroborate Salts

Published on: February 24, 2015

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Published on: June 20, 2014

Area of Science:

Medicinal Chemistry
Molecular Pharmacology
Synthetic Organic Chemistry

Background:

YM-254890 and FR900359 are key inhibitors of the Gq/11-signaling pathway.
These inhibitors are valuable tools for biological research but challenging to synthesize.
Existing synthetic methods lack versatility for analog creation and scalability.

Purpose of the Study:

To develop a versatile and efficient synthetic strategy for YM-254890 and FR900359 analogs.
To enable the rapid construction of diverse non-natural and labeled analogs.
To increase the availability of these important signaling pathway inhibitors.

Main Methods:

A convergent building block approach was employed.
This strategy facilitates modular synthesis.
Key intermediates were synthesized and coupled efficiently.

Main Results:

The developed method allows for the rapid synthesis of various non-natural analogs.
Labeled analogs can be readily prepared using this approach.
The strategy overcomes limitations of previous synthetic routes.

Conclusions:

The novel convergent synthesis provides a versatile platform for generating YM-254890 and FR900359 analogs.
This approach will accelerate research into the Gq/11-signaling pathway.
The method facilitates the production of customized inhibitors for biological investigation.