Related Experiment Video

Updated: Jul 6, 2025

Author Spotlight: THP-1 Macrophage Response to LPS/ATP — Unveiling the Pyroptosis, Apoptosis, and Necroptosis Spectrum
06:12

Author Spotlight: THP-1 Macrophage Response to LPS/ATP — Unveiling the Pyroptosis, Apoptosis, and Necroptosis Spectrum

Published on: May 3, 2024

1.9K

HspBP1 in Complex with the Peptide of the Innate Immunity Protein Tag7 is Able to Lyse Tumor Cells Carrying TNFR1

E A Romanova1, D M Yurkina1, D V Yashin2

  • 1Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.

Doklady. Biochemistry and Biophysics
|January 8, 2024
PubMed

Insights

Researchers discovered that a complex between HspBP1 and a Tag7 peptide (17.1) effectively induces tumor cell death. This finding offers potential for developing novel anti-cancer drugs targeting tumor lysis.

Area of Science:

  • Biochemistry
  • Immunology
  • Oncology

Background:

  • The development of novel cytotoxic agents is crucial for effective cancer therapy.
  • Heat shock proteins and innate immunity proteins play roles in cellular processes.
  • Tag7 protein and its peptide 17.1 are known components of innate immunity.

Purpose of the Study:

  • To investigate the interaction between HspBP1 and the Tag7 protein peptide 17.1.
  • To evaluate the potential of the HspBP1-peptide 17.1 complex as an anti-cancer agent.

Main Methods:

  • Thermophoresis was used to quantify the binding affinity between HspBP1 and Tag7 peptide 17.1.
  • Experiments were conducted to assess the effect of the complex on tumor cells.

Main Results:

  • HspBP1 forms a complex with Tag7 peptide 17.1.
  • The affinity of Tag7 peptide 17.1 for HspBP1 is 100-fold higher than that of the full-sized Tag7 protein.
  • The 17.1-HspBP1 complex induces apoptosis and necroptosis in tumor cells.

Conclusions:

  • The HspBP1-Tag7 peptide 17.1 complex demonstrates significant cytotoxic activity against tumor cells.
  • This complex holds promise for the development of new anti-tumor therapeutics.
Keywords:
17.1 peptideHspBP1Tag7cytotoxicitytumor cells

More Related Videos

Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology08:27

Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology

Published on: March 24, 2015

14.8K
Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection07:54

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection

Published on: September 2, 2014

47.1K

Related Concept Videos

The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.4K
Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
1.7K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
8.9K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.6K
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
921
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.4K