Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PE-STAR: prime editing with SOS-triggered and RecJ-augmented repair enables high-efficiency editing in Escherichia coli.

Nucleic acids research·2026
Same author

Hierarchical Logic Control via DNA Polymerase-Driven Molecular Circuits.

ACS applied bio materials·2026
Same author

Conformation-programmed DNA computing.

Science advances·2026
Same author

DNA-based cooperative games: an interactive collective decision-making architecture.

Organic & biomolecular chemistry·2026
Same author

Engineering <i>Escherichia coli</i> for robust Co-utilization of glucose and xylose enables high-titer succinate production from lignocellulosic hydrolysates.

Synthetic and systems biotechnology·2026
Same author

Integrated dynamic control and enzyme co-localization strategies enable high-efficiency stilbenoid biosynthesis.

Bioresource technology·2026

Related Experiment Video

Updated: Jul 6, 2025

Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates
09:17

Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates

Published on: March 5, 2019

8.6K

Catalytic Gold Nanoparticle Assembly Programmed by DNAzyme Circuits.

Ranfeng Wu1, Yiming Chen2, Yongpeng Zhang3

  • 1School of Computer Science and Technology, Dalian University of Technology, Dalian, 116024, China.

Small (Weinheim an Der Bergstrasse, Germany)
|January 9, 2024
PubMed
Summary

This study introduces a catalytic method using DNAzyme circuits for gold nanoparticle (AuNP) assembly. This approach enables efficient AuNP superstructure formation with minimal DNA triggers, advancing biosensing and nanomedicine applications.

Keywords:
DNA nanotechnologyDNAzyme circuitcatalytic assemblynanoparticle assembly

More Related Videos

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

14.6K
Author Spotlight: Advancements in DNA Nanosensors &#8211; Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

999

Related Experiment Videos

Last Updated: Jul 6, 2025

Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates
09:17

Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates

Published on: March 5, 2019

8.6K
Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

14.6K
Author Spotlight: Advancements in DNA Nanosensors &#8211; Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

999

Area of Science:

  • Nanotechnology and Materials Science
  • Biochemistry and Molecular Biology
  • Chemical Engineering

Background:

  • Assembled gold nanoparticle (AuNP) superstructures exhibit unique properties for diverse applications.
  • Existing DNA-assisted AuNP assembly methods often require non-catalytic, equimolar conditions, posing challenges in complex biological environments and at low trigger concentrations.

Purpose of the Study:

  • To develop a catalytic method for programming AuNP assemblies using DNAzyme circuits.
  • To enable efficient AuNP assembly with significantly reduced DNA trigger concentrations.
  • To explore advanced functionalities like cascading and feedback in catalytic AuNP assembly systems.

Main Methods:

  • Development and implementation of DNAzyme circuits for catalytic control of AuNP assembly.
  • Experimental verification of logic DNAzyme circuits to govern catalytic AuNP assembly.
  • Establishment of catalytic AuNP assembly systems with cascading and feedback mechanisms.

Main Results:

  • A catalytic method was successfully developed, allowing a small number of DNA triggers to induce the production of a large quantity of desired AuNP assemblies.
  • The feasibility of using logic DNAzyme circuits to control catalytic AuNP assembly was experimentally confirmed.
  • Catalytic AuNP assembly systems exhibiting cascading and feedback functions were successfully established.

Conclusions:

  • The study presents a novel catalytic approach for DNA-programmed gold nanoparticle assembly.
  • This method overcomes limitations of non-catalytic approaches, enabling efficient assembly with low DNA trigger concentrations.
  • The developed system offers a promising alternative for nanoparticle assembly, enhancing tools for biosensing and nanomedicine.