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Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment
Published on: May 14, 2019
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Establishing Single-Cell Clones from In Vitro-Cultured Circulating Tumor Cells
1Department of Stem Cell Biology and Regenerative Medicine, University of Southern California, Los Angeles, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|January 9, 2024
Abstract
None:
Cancer is a common health problem with more than 90% of deaths due to metastases. Circulating tumor cells (CTCs) contain precursors that can initiate metastases. However, CTCs are rare, heterogeneous, and difficult to expand in culture. We have previously created CTC-derived cell lines from stage IV breast cancer patients. These CTC lines were used to establish single-cell CTC clones using flow cytometry cell sorting.
