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Related Concept Videos

Electrophoresis: Overview01:20

Electrophoresis: Overview

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Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
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Capillary Electrophoresis: Applications01:30

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
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Capillary Electrophoresis: Instrumentation01:20

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Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

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Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
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Overview Of Cell Separation And Isolation01:20

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
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High-Throughput Continuous Free-Flow Dielectrophoretic Trapping of Micron-Scale Particles and Cells in Paper Using

Md Nazibul Islam1, Bhavya Jaiswal1, Zachary R Gagnon1

  • 1Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, Texas 77843, United States.

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Researchers developed a low-cost, paper-based insulator-based dielectrophoresis (iDEP) system for particle manipulation. This innovative approach uses paper

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Area of Science:

  • Biophysics
  • Microfluidics
  • Materials Science

Background:

  • Dielectrophoresis (DEP) uses non-uniform electric fields for particle manipulation.
  • Insulator-based DEP (iDEP) employs insulating structures to create electric field gradients.
  • Traditional iDEP devices face challenges in commercial adoption due to fabrication costs and scalability.

Purpose of the Study:

  • To demonstrate a low-cost, scalable alternative to traditional iDEP devices.
  • To utilize paper's natural porous structures for iDEP applications.
  • To enable portable and accessible bioparticle manipulation.

Main Methods:

  • Paper-based microfluidic channels fabricated from nonwoven fiberglass paper.
  • Application of electric fields perpendicular to particle flow.
  • Utilizing both external pumps and capillary wicking for fluid flow.
  • Micro computed tomography and finite element analysis for computational modeling.

Main Results:

  • Successful trapping and concentration of microparticles using paper-based iDEP.
  • Effective particle manipulation at low applied voltages (as low as 2 V).
  • Demonstration of iDEP using both constant flow and passive capillary flow.
  • Development of a computational model for understanding microscale DEP forces in paper structures.

Conclusions:

  • Paper-based iDEP offers a viable, low-cost alternative for soft matter manipulation.
  • This platform facilitates the development of portable and robust next-generation iDEP systems.
  • Potential applications in sample purification and liquid handling.