Establishment and characterization of the cell-line of a human endometrial carcinoma

A new cell line of a human endometrial carcinoma, designated as NUE-1, has been established in culture and maintained for more than five years. The cell line has a doubling time of 40 h and grows as a confluent monolayer together with a constant population of free-floating cells. Electron microscopically, NUE-1 cells showed poorly differentiated features. Chromosome analysis revealed hyperdiploidy and two marker chromosomes. When cells from passage 6 were injected subcutaneously into nude mice, tumors developed. The histological pattern was indistinguishable from that of the original tumor, and was undifferentiated carcinoma. The cells contained no estrogen or progesterone receptors.