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Updated: Jul 6, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
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Genetic support of the causal association between gut microbiota and peripheral artery disease: a bidirectional

Hongshuo Shi1, Xin Yuan1, Fangfang Wu1

  • 1Department of Peripheral Vascular Surgery, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Aging
|January 10, 2024
PubMed
Abstract

Insights

This study used Mendelian randomization to investigate the gut microbiota

Area of Science:

  • Microbiome Research
  • Genetics
  • Cardiovascular Disease Epidemiology

Background:

  • The precise relationship between gut microbiota composition and peripheral artery disease (PAD) remains incompletely understood.
  • Investigating potential causal links is crucial for understanding PAD pathogenesis.

Purpose of the Study:

  • To explore the potential causal relationship between 211 gut microbial species and peripheral artery disease (PAD) using Mendelian randomization (MR).
  • To determine if these causal effects are bidirectional.

Main Methods:

  • A two-sample Mendelian randomization (MR) analysis was performed using Genome-wide Association Studies (GWAS) summary statistics from the MiBioGen and FinnGen consortia.
  • Sensitivity analyses were conducted to ensure the robustness of the findings.
  • Reverse MR analysis was employed to assess potential inverse causal relationships.

Main Results:

  • The study identified nine specific gut microbial taxa with a potential causal relationship to PAD.
  • Increased risk of PAD was associated with higher abundance of Family XI, genus *Lachnoclostridium*, and genus *Lachnospiraceae* UCG001.
  • Protective associations with PAD risk were observed for class *Actinobacteria*, family *Acidaminococcaceae*, genus *Coprococcus2*, genus *Ruminococcaceae* UCG004, genus *Ruminococcaceae* UCG010, and order NB1n.

Conclusions:

  • This MR study provides evidence supporting a causal link between specific gut microbiota and PAD.
  • The findings identify key bacterial taxa that may influence PAD risk, offering potential targets for future interventions.
  • No significant reverse causal relationship between PAD and the investigated gut microbiota was detected.
Keywords:
Mendelian randomizationcausal effectgenetic associationgut microbiotaperipheral artery disease

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