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Updated: Jul 6, 2025
A Polyaniline-based Sensor of Nucleic Acids
Published on: November 1, 2016
Imine-linked covalent organic framework with high crystallinity for constructing sensitive purine bases
Hao Guo1, Zeyun Yang1, Lei Sun1
1Key Laboratory of Eco-functional Polymer Materials of the Ministry of Education, Key Lab of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Gansu International Scientific and Technological Cooperation Base of Water-Retention Chemical Functional Materials, Lanzhou 730070, PR China.
A novel electrochemical sensor utilizing cobalt oxide@covalent organic framework (Co3O4@TADM COF) core-shell nanocomposites and multi-walled carbon nanotubes (MWCNT) was developed for sensitive purine base detection. This sensor platform demonstrates excellent performance in detecting guanine and adenine in DNA extracts.
Area of Science:
- Materials Science
- Electrochemistry
- Nanotechnology
Background:
- Covalent organic frameworks (COFs) offer tunable properties for advanced applications.
- Developing efficient electrochemical sensors for biomolecules is crucial for diagnostics.
Purpose of the Study:
- To synthesize a novel TADM-COF material and create core-shell nanocomposites for electrochemical sensing.
- To develop a highly sensitive electrochemical sensor for simultaneous detection of purine bases (guanine and adenine).
Main Methods:
- Synthesis of TADM-COF and its in-situ growth on oxide particles (SiO2, Fe3O4, Co3O4).
- Fabrication of a Co3O4@TADM COF/MWCNT/GCE electrochemical sensor.
- Electrochemical characterization and performance evaluation for purine base detection.
Main Results:
- Successfully synthesized TADM-COF with high surface area (2597 m2 g-1).
- Co3O4@TADM COF/MWCNT/GCE exhibited synergistic effects, enhancing electrochemical response.
- Achieved wide linear ranges (0.6-180 μM) and low LODs (0.020 μM for G, 0.024 μM for A).
- Demonstrated successful application in detecting purine bases in herring DNA extract.
Conclusions:
- The developed sensor platform offers a general strategy for signal amplification in COF-based electrochemical sensing.
- The synergistic combination of Co3O4, TADM-COF, and MWCNT provides a robust system for purine base detection.
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