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MBIP promotes ESCC metastasis by activating MAPK pathway.

Yanchun Ma1, Yuyan Hua2, XiaoJie Yin2

  • 1Translational Medicine Research Center, Shanxi Medical University, Taiyuan, Shanxi 030001, China; Department of Pathology, College of Basic Medicine, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.

Cellular Signalling
|January 10, 2024
PubMed
Summary

MBIP, a gene linked to cancer susceptibility, is highly expressed in esophageal squamous cell carcinoma (ESCC), promoting tumor invasion and metastasis. This suggests MBIP could be a prognostic biomarker for ESCC patients.

Keywords:
EMTEsophageal squamous cell carcinomaMAPK signaling pathwayMBIP

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Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • MBIP is part of the ATAC complex and a known cancer susceptibility gene.
  • Its specific role and mechanism in ESCC are not well understood.

Purpose of the Study:

  • To investigate the role and molecular mechanism of MBIP in ESCC.
  • To determine if MBIP can serve as a prognostic biomarker for ESCC.

Main Methods:

  • Analysis of TCGA and GEO datasets for MBIP expression in ESCC.
  • Kaplan-Meier analysis for prognosis.
  • In vitro (Transwell) and in vivo (mouse xenograft) assays.
  • RNA-seq, qRT-PCR, and Western blotting to elucidate molecular pathways.

Main Results:

  • MBIP expression is significantly higher in ESCC than normal tissues.
  • High MBIP expression correlates with deeper invasion and poorer prognosis.
  • MBIP overexpression enhances ESCC cell migration and invasion; knockdown inhibits these processes.
  • MBIP promotes EMT via JNK/p38 phosphorylation.

Conclusions:

  • MBIP plays a critical role in ESCC progression and metastasis.
  • MBIP may function as a valuable prognostic biomarker for ESCC.