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pH-Dependent HEWL-AuNPs Interactions: Optical Study.

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Summary
This summary is machine-generated.

Changing pH controls protein corona on gold nanoparticles (AuNPs). Gold nanoparticles stabilize hen egg-white lysozyme (HEWL) at alkaline pH, maintaining its activity. This offers practical applications for monitoring colloidal systems.

Keywords:
aggregationhen egg-white lysozymenano-sized goldnanoparticlespHprotein

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Area of Science:

  • Biophysics
  • Nanotechnology
  • Materials Science

Background:

  • Hen egg-white lysozyme (HEWL) is a model protein.
  • Gold nanoparticles (AuNPs) are widely used in biomedical applications.
  • Understanding protein-nanoparticle interactions is crucial for their effective use.

Purpose of the Study:

  • To investigate the effect of pH on HEWL and AuNP colloids.
  • To study the interaction between HEWL and AuNPs.
  • To identify methods for controlling protein corona formation on AuNPs.

Main Methods:

  • Spectroscopy
  • Spectrofluorometry
  • Dynamic light scattering
  • Refractometry

Main Results:

  • pH is an effective controller of protein corona on AuNPs.
  • pH-induced processes include aggregation-deaggregation of AuNPs and monomerization-dimerization-aggregation of HEWL.
  • A critical transition point at pH 7.5 was observed across all optical methods.
  • AuNPs stabilize HEWL at alkaline pH, preserving its enzymatic activity.

Conclusions:

  • pH modulation offers a simple method to control protein corona on AuNPs.
  • AuNPs can act as stabilizers for HEWL, maintaining its biological function.
  • Optical methods provide accessible tools for monitoring HEWL and AuNP colloidal systems.