Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

20.7K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
20.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A study on the prognosis of patients with sepsis or septic shock based on plasma proenkephalin levels: a meta-analysis.

Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals·2026
Same author

Gut microbiota dysbiosis and short-chain fatty acid alterations in pediatric new-onset type 1 diabetes with ketoacidosis.

Journal of endocrinological investigation·2026
Same author

Deep learning-driven MRI radiomics reveals biological subtypes and predicts recurrence risk in rectal cancer.

NPJ precision oncology·2026
Same author

Integrated stealth-sensing fiber-optic sensor via Dirac-cone phononic crystal.

Optics express·2026
Same author

Optimization strategies for crystal orientation in antimony-based chalcogenide thin-film solar cells.

Nano convergence·2026
Same author

Laparoscopic right hemicolectomy with D3 lymph node dissection and Transanal specimen extraction (NOSES VIIIB) in a young woman: A video vignette.

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2026

Related Experiment Video

Updated: Jul 5, 2025

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.2K

Label-Free DNA Hybridization Detection Using a Highly Sensitive Fiber Microcavity Biosensor.

Yao Wu1, Guiyu Wang1, Xiujuan Yu1,2

  • 1College of Physical Science and Technology, Heilongjiang University, Harbin 150080, China.

Sensors (Basel, Switzerland)
|January 11, 2024
PubMed
Summary

This study presents a novel optical fiber biosensor for ultrasensitive DNA detection. The label-free device offers high sensitivity and specificity, paving the way for advanced diagnostics and biological applications.

Keywords:
DNA hybridization detectionMach–Zehnder interferometerlabel-free biosensoropen cavityoptical fiber sensor

More Related Videos

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

999
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

10.6K

Related Experiment Videos

Last Updated: Jul 5, 2025

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.2K
Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

999
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

10.6K

Area of Science:

  • Photonics and Biosensing
  • Biotechnology and Medical Diagnostics

Background:

  • Label-free biosensing is crucial for real-time biological analysis.
  • Optical fiber interferometers offer high sensitivity for refractive index measurements.

Purpose of the Study:

  • To develop and demonstrate a novel label-free optical fiber biosensor for sensitive DNA detection.
  • To investigate the biosensor's performance in terms of sensitivity, specificity, and repeatability.

Main Methods:

  • Fabrication of a microcavity fiber Mach-Zehnder interferometer using offset splicing of single-mode fibers.
  • Immobilization of probe DNA (pDNA) using APTES for complementary DNA (cDNA) capture.
  • Real-time monitoring of DNA hybridization via changes in the optical signal.

Main Results:

  • Achieved high sensitivity of -17,905 nm/RIU for refractive index measurements.
  • Demonstrated a DNA detection sensitivity of 0.32 nm/fM with a limit of detection as low as 48.9 aM.
  • Confirmed highly repeatable and specific performance for DNA detection.

Conclusions:

  • An easy-to-manufacture, ultrasensitive, and label-free DNA biosensor was successfully developed.
  • The biosensor shows significant potential for applications in medical diagnostics, gene identification, and environmental monitoring.