Related Experiment Video

Updated: Jul 5, 2025

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
09:20

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Published on: May 4, 2021

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miR-129 Regulates Yak Intramuscular Preadipocyte Proliferation and Differentiation through the PI3K/AKT Pathway

Chunyu Qin1,2, Hui Wang2, Jincheng Zhong2

  • 1Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining 810016, China.

International Journal of Molecular Sciences
|January 11, 2024
PubMed

Insights

MicroRNA-129 (miR-129) promotes yak fat cell (adipogenesis) development through the PI3K/AKT pathway. This finding aids in understanding yak fat development and improving beef production.

Area of Science:

  • Molecular Biology
  • Animal Science
  • Biochemistry

Background:

  • MicroRNA-129 (miR-129) is involved in cellular processes like adipogenesis.
  • The specific molecular mechanisms of miR-129 in yak adipogenesis are not fully understood.

Purpose of the Study:

  • To investigate the role of miR-129 in yak intramuscular preadipocytes (YIMAs) adipogenesis.
  • To elucidate the molecular pathway regulated by miR-129 in yak adipogenesis.

Main Methods:

  • Overexpression and interference of miR-129 in YIMAs.
  • Analysis of cell differentiation and proliferation.
  • Assessment of the PI3K/AKT pathway activity, including AKT and p-AKT levels.
  • Use of PI3K inhibitor LY294002 to validate the pathway.

Main Results:

  • miR-129 overexpression enhanced YIMAs differentiation and proliferation.
  • miR-129 interference inhibited YIMAs differentiation and proliferation.
  • miR-129 increased AKT and p-AKT activity, indicating activation of the PI3K/AKT pathway.
  • The PI3K/AKT pathway mediates the effects of miR-129 on YIMAs proliferation and differentiation.

Conclusions:

  • miR-129 promotes yak adipogenesis by activating the PI3K/AKT pathway.
  • This study provides a basis for understanding miR-129's role in yak fat development.
  • Findings may inform strategies to improve meat quality and address obesity in cattle production.
Keywords:
PI3K/AKTadipocytesdifferentiationmiR-129proliferationyak

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