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Essential genes analysis reveals small ribosomal subunit protein eS28 may be a prognostic factor and potential

Chao Liang1,2, Juan Zhou3, Yongjie Wang4

  • 1Department of Musculoskeletal Oncology, Fudan University Shanghai Cancer Center, Shanghai Medical College, Fudan University, Shanghai, 200032, PR China.

Journal of Bone Oncology
|January 11, 2024
PubMed
Abstract

Insights

Researchers identified RPS28 as a key gene in osteosarcoma. Targeting RPS28, or its protein eS28, could be a new strategy to treat this bone cancer and improve patient outcomes.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Osteosarcoma, a primary bone malignancy, has limited targeted therapies, leading to poor patient prognosis.
  • Identifying novel therapeutic targets is critical for advancing osteosarcoma treatment strategies.

Purpose of the Study:

  • To screen the DepMap database for essential genes as potential therapeutic targets in osteosarcoma.
  • To elucidate the biological roles of essential genes and validate promising candidates for osteosarcoma treatment.

Main Methods:

  • Screened DepMap database for essential genes; utilized Gene Set Enrichment Analysis (GSEA) for pathway enrichment.
  • Performed univariate/multivariate Cox and Kaplan-Meier survival analyses on TARGET-OSA and GSE21257-OSA datasets.
  • Conducted in vitro/in vivo experiments and RNA sequencing to assess gene function and molecular mechanisms.

Main Results:

  • Identified 934 essential genes, with significant enrichment in the ribosome pathway (195 genes).
  • RPS28, RPS7, and RPS25 emerged as promising candidates; RPS28 showed high expression linked to poor prognosis.
  • RPS28 knockdown inhibited osteosarcoma cell proliferation, migration, and invasion, affecting the MAPK signaling pathway.

Conclusions:

  • RPS28 is identified as an essential gene for osteosarcoma cell survival.
  • The protein eS28, encoded by RPS28, represents a potential therapeutic vulnerability for osteosarcoma.
Keywords:
CRISPR/Cas9 libraryMAPK signal pathwaysOsteosarcomaRPS28Ribosome biogenesiseS28

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