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Aptamer Clicked Poly(ferrocenylsilanes) at Au Nanoparticles as Platforms with Multiple Function[†].
Tibor G Halmagyi1, Nizar B Alsharif2, Mohamed A Berkal1
1E2S UPPA, CNRS, IPREM, Universite de Pau et des Pays de l'Adour F-, 64053, Pau, France.
Chemistry (Weinheim an Der Bergstrasse, Germany)
|January 11, 2024
Summary
This study introduces a new method to create stable gold nanoparticle (AuNP) biosensors using aptamers and a redox-active polymer. These aptamer-AuNP composites show excellent stability and potential for multifunctional sensing applications.
Area of Science:
- Nanotechnology
- Biochemistry
- Materials Science
Background:
- Aptamers offer specific target sensitivity for biosensing.
- Gold nanoparticles (AuNPs) are effective transducers in biosensor development.
- Combining aptamers and AuNPs creates powerful aptasensor systems.
Purpose of the Study:
- To develop a novel method for functional coupling of thrombin-specific aptamers to AuNPs.
- To utilize an anionic, redox-active poly(ferrocenylsilane) (PFS) polyelectrolyte for this coupling.
- To create a promising aptamer-PFS-AuNP composite for multifunctional sensors.
Main Methods:
- Synthesized AuNPs using PFS as a co-reductant and stabilizer.
- Functionalized AuNPs with thrombin-specific aptamers via the PFS polyelectrolyte.
- Investigated the colloidal stability and redox responsiveness of the aptamer-AuNP composite.
Main Results:
- The aptamer-PFS-AuNP composite demonstrated excellent colloidal stability, even in high ionic strength environments.
- The combined electrosteric stabilizing effects of the aptamer and PFS contributed to enhanced stability.
- Thrombin sensitivity and specificity were successfully demonstrated using the aptamer-AuNP system.
Conclusions:
- The novel aptamer-PFS-AuNP composite offers a stable and versatile platform for biosensing.
- The poly(ferrocenylsilane) polymer plays a crucial role in stabilizing AuNPs and enabling aptamer grafting.
- This system holds promise for the development of future multifunctional biosensors.
