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Universal Biomaterial-on-Chip: a versatile platform for evaluating cellular responses on diverse biomaterial
Abdul Raouf Atif1, Morteza Aramesh2, Sarah-Sophia Carter1
1Division of Biomedical Engineering, Department of Materials Science and Engineering, Science for Life Laboratory, Uppsala University, 751 22, Uppsala, Sweden.
Journal of Materials Science. Materials in Medicine
|January 11, 2024
Summary
The Universal Biomaterial-on-Chip (UBoC) device allows in vitro cell behavior assessment on various biomaterials under dynamic flow. This 3D-printed microfluidic platform enables mechanical stimulation and analysis of cell responses, including calcium signaling.
Area of Science:
- Biomaterials Science
- Microfluidics
- Cellular Biology
Background:
- Microfluidics offers physiologically relevant in vitro cell culture with dynamic flow and shear stress.
- Assessing cellular behavior on diverse biomaterials requires adaptable platforms for mechanical stimulation and monitoring.
Purpose of the Study:
- To introduce the Universal Biomaterial-on-Chip (UBoC) device for evaluating cell response on various biomaterial substrates.
- To demonstrate the UBoC platform's capability for mechanical stimulation and in vitro analysis of cell behavior.
- To explore the UBoC's utility in creating complex multicellular in vitro models.
Main Methods:
- Development of a 3D-printed microfluidic device (UBoC) for biomaterial testing.
- Assessment of cell adhesion and proliferation on diverse biomaterials.
- Application of mechanical stimulation to investigate shear-dependent calcium signaling in pre-osteoblasts.
- Co-culture of multiple cell types to establish dynamic multicellular environments.
Main Results:
- The UBoC device successfully evaluated cell biocompatibility, adhesion, and proliferation on different biomaterials.
- Shear-dependent calcium signaling in pre-osteoblasts was investigated using mechanical stimulation on the UBoC platform.
- The platform demonstrated applicability for creating dynamic multicellular models to study cellular interfaces.
Conclusions:
- The Universal Biomaterial-on-Chip (UBoC) is an adaptable microfluidic tool for in vitro cell behavior evaluation.
- The UBoC platform facilitates the study of biomaterial interactions and cellular responses in dynamic microfluidic environments.
- This technology supports the development of advanced in vitro models for biological process investigation.
