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Updated: Jul 5, 2025

A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
Published on: September 25, 2017
On-column trace-level formation of N-nitrosamine in a liquid chromatography-mass spectrometry analytical system
James R Wickens1, Andrew P Dominey1
1Medicinal Science and Technology, GlaxoSmithKline R&D, Stevenage, UK.
Abstract:
A model reaction between di-n-butylamine and sodium nitrite was studied to investigate trace-level N-nitrosamine formation. Liquid chromatography-mass spectrometry (LC-MS) analysis of kinetic time points from an in-progress reaction showed a systematic offset in nitrosamine concentration between quenched and unquenched samples. By combining samples of amine and nitrite in the needle of the autosampler it was demonstrated that N-nitrosamine was formed in the LC-MS system. Further experimentation indicated that nitrosation was occurring on-column.
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