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Updated: Jul 5, 2025

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Loop-mediated isothermal amplification linked a nanoparticles-based biosensor for detecting Epstein-Barr virus.

Xinggui Yang¹, Xiaoyan Zeng², Junfei Huang¹

¹Guizhou Provincial Center for Disease Control and Prevention, Guiyang, 550004, Guizhou, People's Republic of China.

Applied Microbiology and Biotechnology

|January 12, 2024

Summary

A new diagnostic platform, EBV-LAMAD, rapidly detects Epstein-Barr virus (EBV) using loop-mediated isothermal amplification and a gold nanoparticles biosensor. This simplified assay offers sensitive and reliable EBV infection screening, especially for resource-limited settings.

Keywords:

Diagnosis EBV-LAMAD Epstein-Barr virus Gold nanoparticles–based lateral flow biosensors Loop-mediated isothermal amplification

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Last Updated: Jul 5, 2025

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Published on: November 15, 2017

Area of Science:

Virology
Biotechnology
Medical Diagnostics

Background:

Epstein-Barr virus (EBV) establishes lifelong latent infections in B lymphocytes.
Accurate and rapid diagnostic tools are crucial for managing EBV infections.

Purpose of the Study:

To develop a rapid, sensitive, and reliable diagnostic platform for Epstein-Barr virus (EBV) detection.
To create a simplified assay suitable for clinical settings, including resource-limited regions.

Main Methods:

Development of a novel loop-mediated isothermal amplification (LAMP) assay targeting the EBNA-LP gene.
Integration of LAMP with a gold nanoparticles-based lateral flow biosensor (AuNPs-LFB) for detection (EBV-LAMAD).
Optimization and validation of the EBV-LAMAD assay using various pathogen and whole blood samples.

Main Results:

The EBV-LAMAD assay demonstrated a limit of detection (LoD) of 45 copies/reaction.
The assay successfully detected representative EBV pathogens without cross-reactivity with other organisms.
The entire workflow, from sample preparation to detection, was completed within 70 minutes.

Conclusions:

The EBV-LAMAD assay is a rapid, simplified, sensitive, and reliable method for EBV detection.
This assay presents a competitive diagnostic and screening tool for EBV infection in clinical settings.
The EBV-LAMAD assay is particularly advantageous for basic laboratories in resource-limited areas.