Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

5.7K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
5.7K
Flow Cytometry01:23

Flow Cytometry

13.0K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
13.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

State-of-the-Art Biosensors in China.

Biosensors·2026
Same author

Intelligent label-free droplet microfluidic sorting system for single-cell encapsulation and morphology-guided screening.

Microsystems & nanoengineering·2026
Same author

Flexible Wearable Closed-Loop Diagnostic and Therapeutic System: A New Paradigm for Skin Health Management.

ACS sensors·2026
Same author

[Role of carbohydrate response element-binding protein/hypoxia-inducible factor-1α signaling pathway in sepsis-associated encephalopathy in rats].

Zhonghua wei zhong bing ji jiu yi xue·2026
Same author

<i>Camellia oleifera</i> Seed Cake Extract Ameliorates Hyperuricemia and Renal Injury by Modulating Uric Acid Metabolism, Nrf2/Keap1 Pathway, and NLRP3 Inflammasome in Mice.

Food science & nutrition·2026
Same author

Selective decreases in regulatory B cell (Breg) subsets in active uveitis: IL-10+CD19+CD24hiCD38hiBreg in patients and B10 Breg in mice.

International immunology·2026

Related Experiment Video

Updated: Jul 5, 2025

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells
13:44

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells

Published on: November 10, 2023

1.9K

High-Throughput Sorting and Single-Cell Mechanotyping by Hydrodynamic Sorting-Mechanotyping Cytometry.

Yao Chen1, Chen Ni1, Xiaozhe Zhang1

  • 1School of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing, 211189, China.

Small Methods
|January 12, 2024
PubMed
Summary

This study introduces hydrodynamic sorting-mechanotyping cytometry (HSMC) to efficiently isolate and identify circulating tumor cells (CTCs) from blood. HSMC achieves high accuracy in distinguishing tumor cells from background blood cells.

Keywords:
mechanotyping cytometrymicrofluidicssortingtumor cells

More Related Videos

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.7K
Author Spotlight: Investigating Cellular and Molecular Dynamics During Muscle Regeneration Using Cutting-Edge Single-Cell Technologies
11:02

Author Spotlight: Investigating Cellular and Molecular Dynamics During Muscle Regeneration Using Cutting-Edge Single-Cell Technologies

Published on: December 1, 2023

1.1K

Related Experiment Videos

Last Updated: Jul 5, 2025

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells
13:44

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells

Published on: November 10, 2023

1.9K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.7K
Author Spotlight: Investigating Cellular and Molecular Dynamics During Muscle Regeneration Using Cutting-Edge Single-Cell Technologies
11:02

Author Spotlight: Investigating Cellular and Molecular Dynamics During Muscle Regeneration Using Cutting-Edge Single-Cell Technologies

Published on: December 1, 2023

1.1K

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Microfluidics

Background:

  • Accurate identification of circulating tumor cells (CTCs) is challenging due to interference from numerous background blood cells.
  • Existing methods often lack the throughput or specificity required for clinical applications.

Purpose of the Study:

  • To develop a novel hydrodynamic sorting-mechanotyping cytometry (HSMC) system for high-throughput, label-free isolation and multi-parameter mechanotyping of CTCs.
  • To overcome the limitations of background blood cell interference in CTC detection.

Main Methods:

  • Integration of spiral inertial microfluidics for label-free cell sorting and enrichment.
  • A sorting-concentration chip for cell enrichment followed by a detection chip for hydrodynamic deformation analysis.
  • Multi-parameter mechanotyping to differentiate cell types based on mechanical properties.

Main Results:

  • The HSMC system demonstrated high throughput for both cell sorting and mechanotyping.
  • Successful differentiation and identification of white blood cells (WBCs) and three types of tumor cells (A549, MCF-7, MDA-MB-231).
  • Achieved identification accuracies greater than 94% for WBCs and tumor cells, with a maximum accuracy of 99.2%.

Conclusions:

  • HSMC offers a promising approach for the efficient enrichment and accurate identification of CTCs.
  • The system effectively utilizes cellular mechanical properties for label-free cell analysis.
  • This technology has the potential to advance single-cell mechanical analysis in clinical diagnostics.