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Author Spotlight: High-Sensitivity Tissue Factor Activity Assay for Plasma Diagnosis
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Author Spotlight: High-Sensitivity Tissue Factor Activity Assay for Plasma Diagnosis

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Microparticle-associated tissue factor activity correlates with the inflammatory response in septic disseminated

Shishuai Meng1, Bin Xu2, Wei Yang1

  • 1Intensive Care Unit, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.

Peerj
|January 12, 2024
PubMed
Abstract

Insights

Microparticle-associated tissue factor activity (TF+-MP activity) is elevated in sepsis patients with disseminated intravascular coagulation (DIC), correlating with inflammation. This suggests TF+-MP activity promotes inflammation in septic DIC.

Area of Science:

  • Hematology
  • Critical Care Medicine
  • Immunology

Background:

  • Sepsis frequently leads to disseminated intravascular coagulation (DIC).
  • Microparticles contribute to procoagulant and proinflammatory processes.
  • Understanding the role of microparticle-associated tissue factor activity (TF+-MP activity) in sepsis-induced inflammation is crucial.

Purpose of the Study:

  • To investigate the relationship between TF+-MP activity and the inflammatory response in sepsis patients.
  • To determine if TF+-MP activity is associated with the presence of DIC in sepsis.

Main Methods:

  • Collected blood samples from 31 sepsis patients with DIC and 31 sepsis patients without DIC.
  • Measured TF+-MP activity using a tissue factor-dependent FXa generation assay.
  • Assessed inflammatory markers including leukocytes, neutrophils, procalcitonin, C-reactive protein, Interleukin-1β (IL-1β), and Tumor Necrosis Factor-α (TNF-α).

Main Results:

  • Patients with sepsis and DIC exhibited higher levels of inflammatory markers and TF+-MP activity compared to those without DIC.
  • TF+-MP activity showed a strong correlation with inflammatory response indices and DIC scores in sepsis patients.
  • Elevated leukocytes, neutrophils, procalcitonin, C-reactive protein, IL-1β, and TNF-α were observed in the DIC group.

Conclusions:

  • TF+-MP activity is significantly elevated in sepsis patients with DIC.
  • TF+-MP activity is strongly associated with the inflammatory response in septic DIC.
  • TF+-MP activity may play a key role in exacerbating inflammation during septic DIC.
Keywords:
SepsisClotting disorderDICInflammatory responseTF+-MP activity

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