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Updated: Jul 5, 2025

Competitive Genomic Screens of Barcoded Yeast Libraries
Published on: August 11, 2011
Quantifying orthogonal barcodes for sequence census assays
A Sina Booeshaghi1, Kyung Hoi Joseph Min2, Jase Gehring3
1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, United States.
Summary:
Barcode-based sequence census assays utilize custom or random oligonucloetide sequences to label various biological features, such as cell-surface proteins or CRISPR perturbations. These assays all rely on barcode quantification, a task that is complicated by barcode design and technical noise. We introduce a modular approach to quantifying barcodes that achieves speed and memory improvements over existing tools. We also introduce a set of quality control metrics, and accompanying tool, for validating barcode designs.
Availability And Implementation:
https://github.com/pachterlab/kb_python, https://github.com/pachterlab/qcbc.
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