A facile fluorescence Eu MOF sensor for ascorbic acid and ascorbate oxidase detection

Area of Science:

• Materials Science
• Analytical Chemistry
• Biochemistry

Background:

• Ascorbic acid (AA) and ascorbate oxidase (AAO) play crucial roles in biological systems.
• Accurate detection of AA and AAO is vital for biomedical research and diagnostics.
• Existing detection methods may lack sensitivity or specificity for complex samples.

Purpose of the Study:

• To develop a facile and sensitive fluorescence sensor for detecting ascorbic acid (AA) and ascorbate oxidase (AAO).
• To utilize a Eu³⁺-based metal-organic framework (Eu MOF) for a novel sensing mechanism.
• To validate the sensor's performance in real-world biological samples.

Main Methods:

• Fabrication of a Eu³⁺-based metal-organic framework (Eu MOF).
• Utilizing Ce⁴⁺-induced fluorescence quenching of Eu MOF, reversible by Ce³⁺.
• Developing a dual detection strategy based on fluorescence decrease (for AA) and restoration (for AAO).

Main Results:

• The Eu MOF sensor demonstrated effective fluorescence quenching by Ce³⁺.
• Ascorbic acid (AA) reduced Ce⁴⁺ to Ce³⁺, decreasing Eu MOF fluorescence.
• Ascorbate oxidase (AAO) oxidized AA, preventing Ce⁴⁺ reduction and restoring Eu MOF fluorescence.
• Achieved high sensitivity with limits of detection of 0.32 μM for AA and 1.18 U L^-1 for AAO.
• Successfully applied the sensor for AA and AAO determination in real samples.

Conclusions:

• A novel and sensitive fluorescence-based sensor for AA and AAO detection was successfully developed using Eu MOF.
• The sensor exhibits excellent performance, including high sensitivity and applicability in complex biological matrices.
• This Eu MOF sensor holds significant potential for biomedical applications requiring accurate AA and AAO quantification.