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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

102-Plex Approach for Accurate and Multiplexed Proteome Quantification.

Zhen Wu¹, Xirui Huang¹, Lin Huang¹

¹State Key Laboratory of Genetic Engineering, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, China.

Analytical Chemistry

|January 12, 2024

Summary

This summary is machine-generated.

Researchers developed a super multiplexed proteomic analysis method enabling up to 102 samples per experiment. This advanced technique combines TAG-TMTpro and TAG-IBT16 labeling for enhanced large-scale proteomic studies.

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Area of Science:

Proteomics
Analytical Chemistry
Biochemistry

Background:

Large-scale proteomic analyses require high-throughput methods.
Current hyperplexing techniques allow up to 54 samples per experiment using isotopic and isobaric reagents.

Purpose of the Study:

To develop a super multiplexed approach for analyzing up to 102 samples in a single proteomic experiment.
To evaluate the identification and quantification performance of this novel 102-plex method.

Main Methods:

Combination of TAG-TMTpro and TAG-IBT16 labeling strategies.
Systematic investigation using mixtures of *E. coli* and HeLa peptides.
Assessment of identification and quantification accuracy and reliability.

Main Results:

The proposed 102-plex approach successfully enabled analysis of 102 samples in one experiment.
All labeling series demonstrated accurate and reliable quantification performance.
The combined TAG-IBT16 and TAG-TMTpro labeling expands multiplexing capacity significantly.

Conclusions:

The TAG-IBT16 and TAG-TMTpro combination offers a powerful super multiplexed quantification method for large-scale proteomic analysis.
This approach enhances the capacity for high-throughput proteomic studies.
The method provides a valuable tool for advancing proteomic research.