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Circular RNA hsa_circ_0050386 suppresses non-small cell lung cancer progression via regulating the SRSF3/FN1 axis

Jinbin Chen1,2, Boqi Rao1, Zeqin Huang1

  • 1The State Key Lab of Respiratory Disease, The School of Public Health, Guangzhou Medical University, Guangzhou, 511436, China.

Journal of Translational Medicine
|January 12, 2024
PubMed
Abstract

Insights

Circular RNA hsa_circ_0050386 is downregulated in non-small cell lung cancer (NSCLC), suppressing tumor growth and invasion. This circRNA may serve as a potential biomarker and therapeutic target for NSCLC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Non-small cell lung cancer (NSCLC) is the most common cancer globally, with circular RNAs (circRNAs) playing a key role in its progression.
  • The specific roles and mechanisms of circRNAs in NSCLC development are not fully understood.
  • This study focuses on circRNA hsa_circ_0050386 in NSCLC.

Purpose of the Study:

  • To investigate the expression patterns of circRNA hsa_circ_0050386 in NSCLC.
  • To elucidate the functions and underlying mechanisms of hsa_circ_0050386 in NSCLC progression.
  • To evaluate hsa_circ_0050386 as a potential diagnostic biomarker and therapeutic target for NSCLC.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) for expression analysis.
  • In vitro assays (cell proliferation, migration, invasion, cell cycle, apoptosis) and in vivo xenograft models to assess function.
  • Western blot, pull-down assays, RNA immunoprecipitation (RIP), and mass spectrometry to investigate molecular mechanisms.

Main Results:

  • hsa_circ_0050386 expression was significantly lower in NSCLC tissues compared to normal tissues.
  • Lower hsa_circ_0050386 levels correlated inversely with NSCLC clinical stage, T-stage, and M-stage.
  • hsa_circ_0050386 suppressed NSCLC cell proliferation and invasion in vitro and in vivo.
  • hsa_circ_0050386 interacts with SRSF3 to down-regulate Fibronectin 1 (FN1) expression, inhibiting NSCLC progression.

Conclusions:

  • hsa_circ_0050386 inhibits the malignant behaviors of NSCLC cells by down-regulating FN1 expression.
  • hsa_circ_0050386 holds potential as a biomarker and therapeutic target for NSCLC treatment.
Keywords:
Circular RNA (circRNA)Fibronectin 1 (FN1)Non-small cell lung cancer (NSCLC)Serine and arginine-rich splicing factor 3 (SRSF3)

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