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Updated: Jul 5, 2025

Studying DNA Looping by Single-Molecule FRET
Published on: June 28, 2014
RNA Double-Helix Hybridization Measured by Fluorescence Correlation Spectroscopy
Arne Werner1,2
1Institute for Biochemistry and Molecular Biology, Department of Chemistry, Faculty of Mathematics, Computer Science and Natural Science, Hamburg University, Hamburg, Germany. awerner@ifam.uni-kiel.de.
Abstract:
RNA double-strand hybridization is a key player in gene expression regulation. Single-stranded RNA of up to 300 nucleotides forms Watson-Crick base pairs with complementary messenger RNA. Fluorescence-based single-molecule methods allow to study RNA-RNA interaction under physiological conditions. Here is described, how the dissociation constant of RNA double strands can be determined by applying fluorescence correlation spectroscopy.
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