Related Experiment Video

Updated: Jul 5, 2025

Monitoring Stub1-Mediated Pexophagy
08:26

Monitoring Stub1-Mediated Pexophagy

Published on: May 12, 2023

1.6K

Loss of Mptx2 alters bacteria composition and intestinal homeostasis potentially by impairing autophagy

Weihui Yan1,2,3, Shanshan Chen1,2, Ying Wang1,2,3

  • 1Division of Pediatric Gastroenterology and Nutrition, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Communications Biology
|January 13, 2024
PubMed

Insights

Mucosal pentraxin 2 (Mptx2) is vital for maintaining intestinal homeostasis. Its absence in Paneth cells increases susceptibility to inflammation and infection by disrupting gut bacteria and autophagy.

Area of Science:

  • Gastroenterology
  • Immunology
  • Microbiology

Background:

  • Mucosal pentraxin 2 (Mptx2) is identified as a novel Paneth cell marker in the small intestine.
  • The precise function and regulatory mechanisms of Mptx2 within Paneth cells remain largely unexplored.

Purpose of the Study:

  • To elucidate the role of Mptx2 in intestinal homeostasis and Paneth cell function.
  • To investigate the impact of Mptx2 deficiency on intestinal responses to infection and injury.

Main Methods:

  • Generation and utilization of Mptx2 knockout (Mptx2-/-) mice.
  • Modeling intestinal inflammation and injury using lipopolysaccharide (LPS), methicillin-resistant Staphylococcus aureus (MRSA) infection, and dextran sulfate sodium (DSS).

Main Results:

  • Mptx2 is selectively expressed in mouse small intestinal Paneth cells.
  • Mptx2-/- mice exhibit heightened susceptibility to intestinal inflammation, injury, and MRSA infection.
  • Mptx2 deficiency leads to reduced Paneth cell numbers, decreased antimicrobial factor expression, altered gut microbiota, and impaired Paneth cell autophagy.
  • Mptx2 deficiency exacerbates LPS-induced intestinal issues, potentially by inhibiting the autophagy/endoplasmic reticulum (ER) stress pathway.

Conclusions:

  • Mptx2 plays a critical role in maintaining intestinal homeostasis.
  • Mptx2 is essential for Paneth cell function, potentially through the regulation of autophagy.

More Related Videos

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment08:33

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

Published on: November 17, 2018

13.0K
Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions12:52

Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions

Published on: September 9, 2014

15.9K

Related Concept Videos

Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.2K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.6K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Autophagic Cell Death01:18

Autophagic Cell Death

Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.4K