Tracking organic matrix in the seashell by elemental mapping under laser-induced breakdown spectroscopy

As a biogenic calcium carbonate, the seashell plays a crucial role in marine environmental studies. In these studies, it is essential to investigate the composition of the seashell. In this study, we used laser-induced breakdown spectroscopy (LIBS) to analyze the elemental composition of cultured scallop-shell (Patinopecten yessoensis), with a specific focus on examining the organic elements (C, N, O, H) to track the shell organic matrix (SOM). Our findings indicate that the seashell organic layer can be accurately identified by referencing the strong emission of nitrogen or the low signal of calcium. To further confirm the presence of this layer, we employed fluorescence spectroscopy, Raman spectroscopy and FTIR spectroscopy. Correlation analysis revealed a strong connection between LIBS emissions (H, O, CC) and seashell organics, as well as demonstrated the presence of organics in metallic emissions (Si, Ba). However, when we conducted elemental mapping on the shell cross-section, the distribution similarity was observed between the elements N, Ba, and Sr. Based on the correlation of organics and the distribution similarity, it is concluded that barium is an element associated with the SOM. These results highlight the potential of LIBS for organic analysis, which can complement traditional seashell analysis.