Tracking organic matrix in the seashell by elemental mapping under laser-induced breakdown spectroscopy

Area of Science:

• Marine Biology
• Geochemistry
• Spectroscopy

Background:

• Seashells are crucial biogenic calcium carbonate structures in marine studies.
• Investigating seashell composition, particularly the organic matrix, is essential for environmental research.

Purpose of the Study:

• To analyze the elemental composition of cultured scallop shells (Patinopecten yessoensis) using LIBS.
• To specifically examine organic elements (C, N, O, H) for tracking the shell organic matrix (SOM).

Main Methods:

• Laser-induced breakdown spectroscopy (LIBS) for elemental analysis.
• Fluorescence, Raman, and FTIR spectroscopy for confirmation.
• Elemental mapping of shell cross-sections.

Main Results:

• LIBS accurately identifies the seashell organic layer via nitrogen emission or low calcium signals.
• Strong correlations found between LIBS emissions (H, O, CC) and seashell organics.
• Barium (Ba) identified as an element associated with the shell organic matrix (SOM) based on distribution and correlation.

Conclusions:

• LIBS is a valuable tool for analyzing organic components in seashells.
• Barium's association with SOM offers new insights into seashell composition.
• LIBS complements traditional methods for seashell analysis.