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Updated: Jul 5, 2025
Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries
Published on: March 31, 2021
Antibacterial activity of mulberry extracts and purified fractions against oral pathogenic bacteria
Takahisa Shirai1, Yutaroh Satoh2, Kazuyuki Ishihara3
1Faculty of Dentistry, Ohu University, 31-1 Misumido, Tomita-cho, Koriyama-city, Fukusima, Japan.
Objectives:
This study aimed to isolate antibacterial compounds active against periodontopathic bacteria from mulberry (Morus alba) leaves.
Methods:
The acetone-soluble fraction of mulberry leaves was extracted from the oil layer by oil/water separation. The extract was purified using silica gel open-column chromatography. The minimum inhibitory concentration (MIC) of the crude extract or purified fractions against Porphyromonas gingivalis was measured at each step.
Results:
The MIC of the crude extract against P. gingivalis was 62.5-125 μg/mL. The fractions showing activity against P. gingivalis were designated Cf K and Cf P. The MICs of Cf K against P. gingivalis, Fusobacterium nucleatum, Prevotella intermedia, and Streptococcus mutans were 6.25 μg/mL, 25 μg/mL, 12.5 μg/mL, and 12.5 μg/mL, respectively. In contrast, the MICs of Cf P against P. gingivalis, F. nucleatum, P. intermedia, and S. mutans were 25.0 μg/mL, >50 μg/mL, 50 μg/mL, and 12.5-25.0 μg/mL, respectively.
Conclusions:
Mulberry leaves contain antibacterial components against periodontopathic bacteria such as P. gingivalis, F. nucleatum, and P. intermedia.
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