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Methods of Nuclear Reprogramming

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...

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Related Experiment Video

Updated: Jul 5, 2025

Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling

Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling

Published on: May 31, 2017

Neuronal Replenishment via Hydrogel-Rationed Delivery of Reprogramming Factors.

Negar Mahmoudi^1,2, Yi Wang^3,4, Niamh Moriarty⁵

¹Laboratory of Advanced Biomaterials, the John Curtin School of Medical Research, Australian National University, Canberra, ACT 2601, Australia.

|January 15, 2024

Summary

Researchers developed a novel hydrogel delivery system for gene therapy to repair central nervous system injuries. This method enhances neural repair by reprogramming astrocytes into neurons, offering a promising avenue for regenerative neuroscience.

Keywords:

direct reprogramming glial scar neural regeneration neurogenesis self-assembling peptide-based hydrogels trans-differentiation

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Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Published on: January 10, 2018

Area of Science:

Neuroscience
Regenerative Medicine
Biomaterials Science

Background:

Central nervous system injuries result in permanent neuronal loss due to limited regeneration capacity.
Reprogramming reactive astrocytes into induced neurons is a potential neural repair strategy.
Challenges exist in viral vector stabilization, targeting, and delivery for neural reprogramming.

Purpose of the Study:

To develop a precise and controlled delivery system for neural reprogramming transgenes.
To enhance the targeting efficiency and reduce immunogenicity of viral vectors for neural repair.
To investigate the efficacy of a self-assembling peptide (SAP) hydrogel for delivering adeno-associated virus (AAV) vectors.

Main Methods:

Utilized a bioinspired self-assembling peptide (SAP) hydrogel for controlled release of a hybrid adeno-associated virus (AAV) vector (AAVDJ).
The AAVDJ vector carried the NeuroD1 neural reprogramming transgene.
Evaluated the system's performance both in vitro and in vivo for neural reprogramming and tissue repair.

Main Results:

In vitro studies confirmed successful induction of neuron formation via morphological, histochemical, and electrophysiological analyses.
In vivo studies demonstrated SAP-mediated delivery of AAVDJ-NeuroD1 induced astrocyte-to-neuron trans-differentiation.
The method effectively reduced glial scarring and improved vector targeting and reprogramming efficiency.

Conclusions:

A novel SAP hydrogel system provides safe and effective delivery of AAV vectors for central nervous system (CNS) injury repair.
This approach mitigates issues associated with high viral dosage, off-target delivery, and immunogenic reactions.
The findings represent a significant advancement in regenerative neuroscience for treating CNS injuries.