Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Agarose Gel Electrophoresis

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Extraction, encapsulation, and bioaccessibility of polyphenols from fruit and vegetable by-products and their health effects: a decade overview.

Critical reviews in food science and nutrition·2026

Same author

On-deck pH measurement with integrated feedback control on a micro-scale platform for monoclonal antibody low pH viral inactivation.

Biotechnology progress·2026

Same author

Hormonal priming with indole-3-acetic acid modulates antioxidant defense, gene expression, and physiological responses under salinity in <i>Limonium</i> spp.

Frontiers in plant science·2026

Same author

Multi-environment phenotyping of ricebean (<i>Vigna umbellata</i> (Thunb.) Ohwi & Ohashi) germplasm and identification of core set for accelerating the crop improvement programs.

Frontiers in plant science·2026

Same author

Long-Distance Trafficking of Mineral Elements From Soil-to-Seed Under Climate Change Impacts Crop Nutritional Quality.

Plant, cell & environment·2026

Same author

Automation of an integrated micro-scale platform for monoclonal antibody process development by incorporation of a depth filter mimic.

Biotechnology progress·2025

Search research articles

Related Experiment Video

Updated: Jul 5, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

SarCTAB: an efficient and cost-effective DNA isolation protocol from geophytes.

Madhushree Dutta^1,2, Paras Sharma¹, Vidhi Raturi^1,2

¹Integrative Plant AdaptOmics Lab (iPAL), Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology (IHBT), Palampur, Himachal Pradesh 176061 India.

|January 15, 2024

Summary

A new SarCTAB DNA extraction method offers an economical, quick, and sustainable solution for isolating high-quality genomic DNA from geophytes, overcoming challenges posed by contaminants.

Keywords:

CTAB DNA isolation Geophytes Polysaccharides Sarcosine Storage organ

More Related Videos

Genotyping of Plant and Animal Samples without Prior DNA Purification

Genotyping of Plant and Animal Samples without Prior DNA Purification

Published on: September 24, 2012

Removal of Exogenous Materials from the Outer Portion of Frozen Cores to Investigate the Ancient Biological Communities Harbored Inside

Removal of Exogenous Materials from the Outer Portion of Frozen Cores to Investigate the Ancient Biological Communities Harbored Inside

Published on: July 3, 2016

Related Experiment Videos

Last Updated: Jul 5, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

Genotyping of Plant and Animal Samples without Prior DNA Purification

Genotyping of Plant and Animal Samples without Prior DNA Purification

Published on: September 24, 2012

Removal of Exogenous Materials from the Outer Portion of Frozen Cores to Investigate the Ancient Biological Communities Harbored Inside

Removal of Exogenous Materials from the Outer Portion of Frozen Cores to Investigate the Ancient Biological Communities Harbored Inside

Published on: July 3, 2016

Area of Science:

Plant molecular biology
Genomics
Biotechnology

Background:

Geophytes are valuable models for studying storage organ formation.
Extracting high-quality genomic DNA from geophytes is challenging due to polysaccharides and polyphenols.
Existing DNA isolation methods are time-consuming and involve multiple steps.

Purpose of the Study:

To develop an economical, quick, and sustainable DNA extraction method for geophytes.
To optimize the traditional CTAB method for geophyte DNA isolation.
To address challenges associated with polysaccharide and polyphenol contamination.

Main Methods:

Modification of the traditional CTAB method using optimized sarcosine, β-mercaptoethanol, and high NaCl concentration.
Evaluation of the SarCTAB method on storage organs of six different geophyte species.
Assessment of DNA quality and yield, and its performance in downstream applications.

Main Results:

The SarCTAB method yielded an average of 1755 ng/µl high-quality DNA from 100 mg of tissue.
Isolated DNA exhibited good purity (260/280 ≈ 1.86, 260/230 ≈ 1.42).
The DNA was suitable for ISSR amplification, restriction digestion, and PCR amplification of barcode genes (matK, rbcL).

Conclusions:

The SarCTAB method is effective for cost-efficient isolation of high-quality genomic DNA from geophyte underground tissues.
This method is suitable for various molecular applications including next-generation sequencing and DNA barcoding.
SarCTAB provides a superior alternative to existing methods for geophyte DNA extraction.