Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Does the substantia nigra have a cholinergic innervation?

Z Henderson, S A Greenfield

    Neuroscience Letters
    |January 14, 1987
    PubMed
    Summary

    Choline acetyltransferase fibers were mapped in the ferret substantia nigra. These nerve fibers, crucial for acetylcholine signaling, were found in axons, not cell bodies, with higher density in the zona compacta.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Water-induced reordering in ultrathin ionic liquid films.

    Journal of physics. Condensed matter : an Institute of Physics journal·2018
    Same author

    An evaluation of in vivo voltage-sensitive dyes: pharmacological side effects and signal-to-noise ratios after effective removal of brain-pulsation artifacts.

    Journal of neurophysiology·2012
    Same author

    Induction of θ-frequency oscillations in the rat medial septal diagonal band slice by metabotropic glutamate receptor agonists.

    Neuroscience·2011
    Same author

    The membrane chamber: a new type of in vitro recording chamber.

    Journal of neuroscience methods·2010
    Same author

    Environmental enrichment differentially modifies specific components of sensory-evoked activity in rat barrel cortex as revealed by simultaneous electrophysiological recordings and optical imaging in vivo.

    Neuroscience·2010
    Same author

    Measurement of cholinesterase activity released from the brain "on-line" and in vivo.

    Neurochemistry international·2010

    Area of Science:

    • Neuroscience
    • Neuroanatomy

    Background:

    • The substantia nigra is a key brain region involved in motor control and reward.
    • Choline acetyltransferase (ChAT) is the enzyme responsible for synthesizing acetylcholine, a vital neurotransmitter.

    Purpose of the Study:

    • To investigate the distribution of choline acetyltransferase-containing fibers within the ferret substantia nigra.
    • To determine the precise location and density of cholinergic projections in different substantia nigra layers.

    Main Methods:

    • Immunohistochemistry was employed to visualize choline acetyltransferase.
    • Light microscopy was used to examine the stained fibers at the cellular level in ferret brain tissue.

    Main Results:

    • Choline acetyltransferase staining was absent in substantia nigra cell bodies.
    • A network of fine, varicose axons containing choline acetyltransferase was identified.
    • These cholinergic fibers were more densely distributed in the zona compacta compared to the zona reticulata.

    Conclusions:

    • The ferret substantia nigra receives cholinergic innervation primarily through axonal projections, not local cell bodies.
    • The differential distribution suggests a specific role for cholinergic input in the zona compacta of the substantia nigra.

    Related Experiment Videos