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Updated: Jul 5, 2025

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool
Published on: May 25, 2021
Development of an analytical method to determine methyl alcohol and isopropyl alcohol in food using static headspace
Heung Youl Lim1, Mi-Hyun Ka1, Nae In Chang1
1Korea Health Supplements Association Sub. Korea Health Supplements Institute, Gyeonggi-do, Seongnam-si, 13488 South Korea.
Abstract:
A method to determine methanol (MeOH) and isopropyl alcohol (IPA) was developed using static headspace sampling (HSS) with gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The optimized HSS-GC and HSS-GC/MS methods were validated according to the parameters. The limit of quantification (LOQ) values for MeOH analyzed by HSS-GC/MS and HSS-GC were 0.50-0.56 and 1.97 mg/L, and the corresponding values for IPA were 0.14-0.21 and 1.51 mg/L, respectively. Recoveries were determined by spiking sample matrices, such as vinegar and tea. MeOH and IPA showed excellent recoveries of 93.80-107.60% and 93.92-104.32% with corresponding precisions of 0.51-7.38% and 0.90-7.70%, respectively. Compared to solid-phase microextraction-GC/MS and HSS-GC, the HSS-GC/MS method showed outstanding values for limit of detection and LOQ with improved precision and accuracy. This HSS-GC/MS method was successfully used to monitor residual solvents in 100 food and functional food products.
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