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Diagnostic and prognostic value of c-MYC gene expression in hematological malignancies
Ifeyinwa Maryann Okafor1, Henshaw Uchechi Okoroiwu1,2, Christopher Ogar Ogar1
1Department of Haematology and Blood Transfusion Science, Faculty of Medical Laboratory Science, University of Calabar, Nigeria.
Introduction:
c-MYC plays vital role in regulation of cell proliferation and has been associated with tumorigenesis. This study is aimed at assessing diagnostic and prognostic value of plasma c-MYC expression to aid in early diagnosis and prognosis of hematological malignancies.
Methods:
Plasma c-MYC expression was determined by quantitative real time PCR using EVA Green chemistry and cluster of differentiation markers performed via immunocytochemistry.
Result:
Plasma c-MYC was higher in subject with hematological malignancies (8.8 ± 1.1) when compared with apparently healthy controls (4.5 ± 0.5). A screening cut-off c-MYC ratio value of 9.42 with sensitivity and specificity of 65.5% and 100% respectively were obtained using receiver operator characteristic curve analysis. Plasma c-MYC was found to have no prognostic value using Kaplan-Meier analysis.
Conclusion:
Plasma c-MYC ratio showed promising screening/diagnostic value for hematological malignancies.
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