Automated hyperspectral imaging for non-invasive characterization of human eye vasculature: A potential tool for...

The vascular supply to the human eye plays a vital role in maintaining ocular health, making its non-invasive evaluation essential for diagnosing and managing various ocular disorders. This paper presents a novel approach utilizing hyperspectral imaging (HSI) to non-invasively characterize human eye vasculature. The proposed system aims to specifically identify the blood atrium and veins of the human eye at 470 nm and 750 nm, respectively, using quantitative phase analysis and k-means clustering. The study involved capturing diffused reflection spectra and hyperspectral images of the human eye at different wavelengths to reveal distinctive vascular features. The results of ten volunteers demonstrate promising capabilities in automated differentiation of atrium and veins, as well as the potential for mapping varicose veins in the lower limb. This non-invasive and non-contact imaging technique shows great promise in facilitating accurate and detailed evaluation of ocular blood flow, providing valuable information for clinical diagnosis and treatment in ophthalmology and vascular medicine fields.