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Whole-genome CRISPR screens to understand Apicomplexan-host interactions
Eva Hesping1,2, Justin A Boddey1,2
1Infectious Diseases and Immune Defence Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.
Molecular Microbiology
|January 15, 2024
Summary
CRISPR/Cas9 technology revolutionizes functional genomics in Apicomplexan parasites, aiding the discovery of drug targets for diseases. This review highlights key findings and future challenges in parasite-host interaction studies.
Area of Science:
- Parasitology
- Genomics
- Molecular Biology
Background:
- Apicomplexan parasites cause significant human and livestock diseases.
- Understanding parasite biology is crucial for developing therapeutic interventions.
- Functional genomics is key to identifying drug targets.
Purpose of the Study:
- To review key discoveries from CRISPR/Cas9 screens in Apicomplexa.
- To discuss the application of CRISPR/Cas9 in studying parasite-host interactions.
- To identify challenges and future directions for CRISPR/Cas9 technology in Apicomplexa.
Main Methods:
- Utilizing CRISPR/Cas9 for forward genetics and whole-genome screens.
- Analyzing gene function during Apicomplexan infection of host cells.
- Highlighting discoveries from recent CRISPR/Cas9 screens.
Main Results:
- CRISPR/Cas9 screens have significantly advanced functional gene analysis in Apicomplexa.
- This technology has enabled deeper insights into parasite-host interactions.
- Key discoveries have identified potential therapeutic targets.
Conclusions:
- CRISPR/Cas9 technology is a powerful tool for Apicomplexan functional genomics.
- Further optimization is needed to fully leverage CRISPR/Cas9 for parasite research.
- Continued application will enhance understanding of parasite-host interactions and disease control.
