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Updated: Jul 5, 2025
A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
Published on: April 18, 2025
Proteomic Difference Analysis of Whole Blood and Bloodstains
Ao Huang1,2, Shu-Bo Wen1,2, Qian-Qian Kong2
1School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, Jiangsu Province, China.
Objectives:
To study the changes of protein levels in peripheral blood after it dried.
Methods:
The proteins from whole blood and bloodstains were detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and normalized by the label-free quantification (LFQ) method. The differential proteins were analyzed by using R 4.2.1 software, limma and edgeR package. The analysis of biological function, signaling pathway and subcellular localization for the differential proteins was then performed.
Results:
A total of 623 and 596 proteins were detected in whole blood and bloodstains, respectively, of which 31 were statistically significant in the quantitative results, including 10 up-regulated and 21 down-regulated proteins in bloodstains.
Conclusions:
The protein abundances in whole blood and bloodstains are highly correlated, and the variation of protein abundances may be related to the changes of endogenous and structural proteins in cells. The application of proteomics technology can assist the screening and identification of protein biomarkers, thereby introducing new biomarkers for forensic research.
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