Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

199
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
199
Atomic Force Microscopy01:08

Atomic Force Microscopy

3.4K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.4K
Properties of Fourier Transform I01:21

Properties of Fourier Transform I

176
The application of Fourier Transform properties in radio broadcasting is multifaceted, enabling significant advancements in the way signals are transmitted and received. Key areas where these properties are utilized include simultaneous multi-channel transmission, audio clip speed adjustments, live broadcast delays for different time zones, audio frequency adjustments, and signal demodulation.
In radio broadcasting, multiple audio signals often need to be transmitted simultaneously. The Fourier...
176
Properties of Fourier Transform II01:24

Properties of Fourier Transform II

217
The Fourier Transform (FT) is an essential mathematical tool in signal processing, transforming a time-domain signal into its frequency-domain representation. This transformation elucidates the relationship between time and frequency domains through several properties, each revealing unique aspects of signal behavior.
The Frequency Shifting property of Fourier Transforms highlights that a shift in the frequency domain corresponds to a phase shift in the time domain. Mathematically, if x(t) has...
217

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Counting particles in cryo-electron microscopy may result in incorrect population estimates.

Communications biology·2026
Same author

Bayesian perspective for orientation determination in cryo-EM with application to structural heterogeneity analysis.

Acta crystallographica. Section D, Structural biology·2026
Same author

Bayesian Perspective for Orientation Determination in Cryo-EM with Application to Structural Heterogeneity Analysis.

bioRxiv : the preprint server for biology·2025
Same author

The Inaugural Flatiron Institute Cryo-EM Conformational Heterogeneity Challenge.

bioRxiv : the preprint server for biology·2025
Same author

Multi-target detection with application to cryo-electron microscopy.

Inverse problems·2025
Same author

Cryo-EM heterogeneity analysis using regularized covariance estimation and kernel regression.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Jul 5, 2025

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

4.9K

Self Fourier shell correlation: properties and application to cryo-ET.

Eric J Verbeke1, Marc Aurèle Gilles2, Tamir Bendory3

  • 1Program in Applied and Computational Mathematics, Princeton University, Princeton, NJ, USA. ev9102@princeton.edu.

Communications Biology
|January 16, 2024
PubMed
Summary

This study presents a method to estimate the Fourier shell correlation (FSC) from a single measurement, expanding its use in microscopy. The approach enables resolution estimation and denoising for structural biology applications.

More Related Videos

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
08:47

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

Published on: March 15, 2021

3.9K
Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography
08:20

Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography

Published on: October 25, 2021

3.3K

Related Experiment Videos

Last Updated: Jul 5, 2025

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

4.9K
A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
08:47

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

Published on: March 15, 2021

3.9K
Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography
08:20

Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography

Published on: October 25, 2021

3.3K

Area of Science:

  • Microscopy
  • Structural Biology
  • Image Analysis

Background:

  • Fourier shell correlation (FSC) is vital for assessing image quality and resolution in microscopy.
  • Current FSC methods typically require two independent measurements, limiting their application.

Purpose of the Study:

  • To develop and validate a method for estimating FSC from a single measurement.
  • To extend the applicability of FSC analysis in microscopy, particularly in structural biology.

Main Methods:

  • Derivation of conditions for estimating FSC from downsampled, noisy single measurements.
  • Implementation of corrections to enhance the method's robustness.
  • Application to estimate global resolution from 3-D structures and denoise electron cryo-tomography data.

Main Results:

  • Successfully estimated FSC from single measurements under derived conditions.
  • Demonstrated accurate global resolution estimation from a single 3-D structure.
  • Showcased effective denoising of tomographic reconstructions using the single-measurement FSC approach.

Conclusions:

  • The developed method expands FSC applications by utilizing single measurements.
  • Provides guidelines for single-measurement FSC computation and suggests new uses in microscopy.
  • Enhances resolution determination and denoising in structural biology and cryo-electron tomography.